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Cy5 alkyne (non-sulfonated)
作者：德尔塔 日期：2022-09-27
used for Click chemistry to label any molecule bearing azide group.Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Various substrates such as biomolecules, polymers, and solid surfaces which bearing azides can be used for the labeling. In the azido-GalNAc samples, alkyne-Cy3 and alkyne-Cy5 specifically label azido-proteins. Furthermore, researches also indicate that the Cy5 and Cy3 probe-protein conjugates have non-overlapping fluorescence profiles and can be differentially detected within a single gel [1].Reference:[1] Burnham-Marusich, A. R.; Plechaty, A.M.; Berninsone, P.M. Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation. Electrophoresis, 2014, 35(18), 2621-2625.Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides
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Cy5 (triethylamine salt)
作者：德尔塔 日期：2022-09-27
Cy5 is a cyanine-containing fluorochrome.1 It has commonly been conjugated to secondary antibodies for use in immunocytochemistry (ICC) and immunohistochemistry (IHC) applications. Cy5 displays excitation/emission maxima of 650/670 nm, respectively.1.Suzuki, T., Matsuzaki, T., Hagiwara, H., et al.Recent advances in fluorescent labeling techniques for fluorescence microscopyActa Histochem. Cytochem.40(5)131-137(2007)Cy5 is a cyanine-containing fluorochrome.1 It has commonly been conjugated to secondary antibodies for use in immunocytochemistry (ICC) and immunohistochemistry (IHC) applications. Cy5 displays excitation/emission maxima of 650/670 nm, respectively.1.Suzuki, T., Matsuzaki, T., Hagiwara, H., et al.Recent advances in fluorescent labeling techniques for fluorescence microscopyActa Histochem. Cytochem.40(5)131-137(2007)Cas No. N/A SDF 别名 N/A 化学名 N/A Canonical SMILES OC(CCCCCN1/C(C(C)(C2=C1C=CC(S(O)(=O)=O)=C2)C)=C/C=C/C=C/C3=[N+](C4=CC=C(C=C4C3(C)C)S([O-])(=O)=O)CC)=O.CCN(CC)CC 分子式 C33H40N2O8S2.C6H15N 分子量 758 溶解度 DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: 30 mg/ml,PBS (pH 7.2): 10 mg/ml 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in
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CY5
作者：德尔塔 日期：2022-09-27
Cy5是一种用于标记肽, 蛋白质, 寡核苷酸的氨基基团的反应染料。激发波长 (nm): 649, 发射波长 (nm): 670。Cy5 is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling.Excitation (nm):649, Emission (nm): 670.Cy5 is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling.Excitation (nm):649, Emission (nm): 670.Cas No. 146368-11-8 SDF 别名 N/A 化学名 N/A Canonical SMILES CC(/C(N1CCCCCC(O)=O)=C\C=C\C=C\C2=[N+](CC)C(C=CC(S(=O)([O-])=O)=C3)=C3C2(C)C)(C)C4=C1C=CC(S(=O)(O)=O)=C4 分子式 C33H40N2O8S2 分子量 656.81 溶解度 H2O: 25 mg/mL (38.06 mM) 储存条件 Store at -20°C,protect from light
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Cy3.5 NHS ester (non-sulfonated)
作者：德尔塔 日期：2022-09-27
Labeling of amino-groups in biomolecules.Cyanine3.5 NHS ester is a dye for labeling of oligonucleotides, peptides and proteins which contain amino-groups. For fluorescence imaging and other fluorescence-based biochemical analysis, it has been used to label biological molecules too. Cy3.5 dyes have enhanced fluorescence upon binding to proteins. The excitation peak and emission peak of Cy3.5 dye is around 550nm and 590nm respectively. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. The ENU cells immersed in 0.3 mM Cy3.5 NHS ester with modified balanced salt solution (MBSS) showed that Cy3.5 NHS ester does not label the dead cells [1].Reference:1. XueYi Xie. Tumor angiogenesis, O2 saturation, glucose and amino acid metabolisms study using functional imaging. PhD thesis.Cyanine3.5 NHS ester is a dye for labeling of oligonucleotides, peptides and proteins which contain amino-groups. For fluorescence imaging and other fluorescence-based biochemical analysis, it has been used to label biological molecules too. Cy3.5 dyes have enhanced fluorescence upon binding to proteins. The excitation peak and emission peak of Cy3.5 dye is around 550nm and 590nm respectively. Most derivatives of non-sulfonated cyanines have
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Cy3.5 maleimide (non-sulfonated)
作者：德尔塔 日期：2022-09-27
Labeling of cysteine residues, as well as other thiolated moleculesCy3.5 maleimide can be used for selective labeling of free sulfhydryl groups (e.g. cysteine residues in peptides, proteins and oligonucleotides). Before labeling, cystines should be prepared with reductant such as TCEP (tris-carboxyethylphosphine). The mono-reactive malemides is particularly good for the labeling of antibody, that’s because the dye molecules are located away from the antibody binding site and functioning of the labeled molecules can be protected.Cy3.5 maleimide can be used for selective labeling of free sulfhydryl groups (e.g. cysteine residues in peptides, proteins and oligonucleotides). Before labeling, cystines should be prepared with reductant such as TCEP (tris-carboxyethylphosphine). The mono-reactive malemides is particularly good for the labeling of antibody, that’s because the dye molecules are located away from the antibody binding site and functioning of the labeled molecules can be protected.Cas No. N/A SDF 化学名 3-(6-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)amino)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E)-3-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)prop-1-en-1-yl)-1H-benzo[e]indol-3-ium chloride Canonical SMILES CN1C2=CC=C3C=CC=CC3=C2C(C)(C)/C1=C\C=C\C4=[N+](CCCCCC(NCCN5C(C=CC5=O)=O)=O)C6=CC=C(C=CC=C7)C7=C6C4(C)C.[Cl-] 分子式 C44H47ClN4O3 分子量 71
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Cy3.5 hydrazide (non-sulfonated)
作者：德尔塔 日期：2022-09-27
carbonyl-reactive dyeCy3.5 hydrazide is a near infrared fluorophore that can react with various carbonyl groups in biomolecules which are generated after oxidative stress or deamination of proteins. In addition, aldehyde moieties can be produced by periodate oxidation of sugar residues of glycoproteins, such as antibodies that can then be oxidized by sodium periodate and labeled with Cy3.5 hydrazide to form Cy3. 5 labeled antibodies. For biomolecule labeling, the labeling regent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.Cy3.5 hydrazide is a near infrared fluorophore that can react with various carbonyl groups in biomolecules which are generated after oxidative stress or deamination of proteins. In addition, aldehyde moieties can be produced by periodate oxidation of sugar residues of glycoproteins, such as antibodies that can then be oxidized by sodium periodate and labeled with Cy3.5 hydrazide to form Cy3. 5 labeled antibodies. For biomolecule labeling, the labeling regent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.Cas No. N/A SDF 化学名 3-(6-hydrazi
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Cy3.5 azide (non-sulfonated)
作者：德尔塔 日期：2022-09-27
used for Click chemistry to label alkyne-modified oligonucleotidesCy3.5 azide is a solid material dye ready for the use in Click Chemistry reaction. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Cy3.5 azide could easily interfere with a radical polymerisation process as it contains a variety of double bonds. The Cy-3 modified initiator was then used to grow the modified PMPC-PDPA directly from the dye [1].Reference:[1] Gaitzsch, J. ; Delahaye, M.; Poma, A.; Du Prez, F.; Battaglia, G. Comparison of metal free polymer-dye conjugation strategies in protic solvents. Polymer Chemistry, 2016, 7(17), 3046–3055.Cy3.5 azide is a solid material dye ready for the use in Click Chemistry reaction. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Cy3.5 azide could easily interfere with a radical polymerisation process as it contains a variety of double bonds. The Cy-3 modified initiator was then used to grow the modified PMPC-PDPA directly fro
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Cy3.5 alkyne (non-sulfonated)
作者：德尔塔 日期：2022-09-27
用于点击化学反应的染料炔Cy3.5 alkyne (non-sulfonated)是一种荧光基团，可以通过铜催化的点击化学反应和叠氮化物反应。它尤其适合在叠氮化钠-炔的环化作用之后，通过荧光光谱来检测和标记含叠氮化钠分子/生物分子。这种染料是非水溶性的，但是可以通过有机助溶剂（DMSO或DMF）来有效的和水溶性分子（比如蛋白或多肽）结合。Cy3.5 alkyne (non-sulfonated)是一种荧光基团，可以通过铜催化的点击化学反应和叠氮化物反应。它尤其适合在叠氮化钠-炔的环化作用之后，通过荧光光谱来检测和标记含叠氮化钠分子/生物分子。这种染料是非水溶性的，但是可以通过有机助溶剂（DMSO或DMF）来有效的和水溶性分子（比如蛋白或多肽）结合。Cas No. N/A SDF 别名 N/A 化学名 N/A Canonical SMILES CC(C1=C(C=CC=C2)C2=CC=C1N/3C)(C)C3=C/C=C/C4=[N+](CCCCCC(NCC#C)=O)C(C=CC5=C6C=CC=C5)=C6C4(C)C.[Cl-] 分子式 C41H44ClN3O 分子量 630.26 溶解度 >63 mg/mL in DMSO; insoluble in H2O; >50.5 mg/mL in EtOH 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
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CY3-YNE
作者：德尔塔 日期：2022-09-27
CY3-YNE是一种染料,可用于标记可溶性蛋白、肽和寡核苷酸/DNA。CY3-YNE is a dye for the labeling of soluble proteins, peptides, and oligonucleotides/DNA.CY3-YNE is a dye for the labeling of soluble proteins, peptides, and oligonucleotides/DNA.Cas No. 1010386-62-5 SDF 别名 N/A 化学名 N/A Canonical SMILES CC(/C(N1CCCCCC(NCC#C)=O)=C\C=C\C2=[N+](CC)C(C=CC(S(=O)(O)=O)=C3)=C3C2(C)C)(C)C4=C1C=CC(S(=O)(O)=O)=C4 分子式 C34H42N3O7S2 分子量 668.84 溶解度 DMSO : ≥ 30 mg/mL (44.85 mM) 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
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CY3-SE
作者：德尔塔 日期：2022-09-27
Cy3-SE是一种反应染料, 为氨基基团的的生物分子标记。Cy3-SE可用于可溶性蛋白, 肽和寡核苷酸/DNA的标记。激发波长 (nm):555，发射波长 (nm): 570。CY3-SE is a reactive dye for the labeling of amino-groups in biomolecules. CY3-SE is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. Excitation (nm):555, Emission (nm): 570.CY3-SE is a reactive dye for the labeling of amino-groups in biomolecules. CY3-SE is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. Excitation (nm):555, Emission (nm): 570.Cas No. 146368-16-3 SDF 别名 N/A 化学名 N/A Canonical SMILES CC(/C(N1CCCCCC(ON2C(CCC2=O)=O)=O)=C\C=C\C3=[N+](CC)C(C=CC(S(=O)([O-])=O)=C4)=C4C3(C)C)(C)C5=C1C=CC(S(=O)(O)=O)=C5 分子式 C35H41N3O10S2 分子量 727.84 溶解度 DMSO: ≥ 62.5 mg/mL (85.87 mM) 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
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Cy3-N3
作者：德尔塔 日期：2022-09-27
Cy3-N3 是 Cy3 叠氮化物荧光染料，用于多肽、蛋白和寡核苷酸中的氨基。Cy3-N3 is a Cy3-azide fluorescent dye used to label for protein and nucleic acid.[1]. Palsuledesai CC, et al. A combination of metabolic labeling and 2D-DIGE analysis in response to a farnesyltransferase inhibitor facilitates the discovery of new prenylated proteins.Cy3-N3 is a Cy3-azide fluorescent dye used to label for protein and nucleic acid.[1]. Palsuledesai CC, et al. A combination of metabolic labeling and 2D-DIGE analysis in response to a farnesyltransferase inhibitor facilitates the discovery of new prenylated proteins.Cas No. N/A SDF 别名 N/A 化学名 N/A Canonical SMILES CC1(C)C(/C=C/C=C2N(CCCCCC(NCCCN=[N+]=[N-])=O)C(C=CC(S(=O)(O)=O)=C3)=C3C\2(C)C)=[N+](CC)C4=CC=C([S-2](=O)(=O)=O)C=C41 分子式 C34H44N6O7S2 分子量 712.88 溶解度 Soluble in DMSO 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
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Cy3 NHS ester(Et)
作者：德尔塔 日期：2022-09-27
氨基反应性荧光染料，溶于水Cas No. N/A SDF 别名 N/A 化学名 N/A Canonical SMILES CC(C1=CC(S(=O)([O-])=[O-])=CC=C1N/2CC)(C)C2=C/C=C/C3=[N+](CCCCCC(ON4C(CCC4=O)=O)=O)C(C=CC(S(=O)([O-])=[O-])=C5)=C5C3(C)C.[Na+] 分子式 C35H40N3NaO10S2 分子量 749.83 溶解度 Soluble in DMSO 储存条件 Store at -20°C General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. Shipping Condition Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
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Cy3 NHS ester (non-sulfonated)
作者：德尔塔 日期：2022-09-27
Labeling of amino-groups in biomolecules.Cyanine, a kind of synthetic dye belonging to the polymethine group, is still used in industry to increase the sensitivity range of photographic emulsions (such as increasing the wavelength range and making the film panchromatic), and in biotechnology more recently. As fluorescent dyes, cyanines have been particularly used in biomedical imaging. Depending on their structure, cyanines can cover the spectrum from UV to IR.Cy3 NHS ester, a reactive dye for labeling of amino-groups in biomolecules, is a Cy3® NHS ester analog. This reagent is commonly used for the labeling of soluble proteins, peptides, and oligonucleotides or DNA. Cy3 NHS ester requires the use of organic co-solvent (such as DMF, or DMSO) for the labeling, however, considering the usage of water-soluble sulfo-Cy3 NHS ester for delicate proteins, such co-solvent is not necessary. Cy3 fluoresce is in the orange region (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the red (~650/670 nm) but absorbs in the orange (~649 nm). Cy3 can be monitored by fluorometers, imagers, and microscopes with standard Tetramethylrhodamine (TRITC) filters. Due to its inherently high extinction coefficient, Cy3 is even detected by naked eye on gels and in solution easily.Reference:[1].http://en. wikipedia.org/wiki/CyanineCyanine, a kind of synthetic dye belonging to the polymethine group, is still used in industry to increase the sensitivity range of photographic emulsions (such as increasing the wavelength range and making the film panchromatic), and in biotechnology more r
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Cy3 NHS ester
作者：德尔塔 日期：2022-09-27
Labeling of amino-groups in biomolecules.Sulfo-Cy3 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For labeling proteins and peptides, no organic co-solvent is needed. Sulfo-Cy3 NHS ester is ideal for proteins with low solubility and proteins prone to denaturation. Non-sulfonated Cy3 NHS ester is also available. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions. In vitro, sulfo-Cy3 NHS ester can react with green-emitting QDs ligand-exchanged and LA/His-PIMA-PEG-NH2 to provide QD-dye conjugates [1].Reference:[1] Reid, D. A.; Keegan, S.; Leo-Macias, A.; Watanabe, G.; Strande, N.T.; Chang, H.H.; Oksuz, B.A.; Fenyo, D.; Lieber, M.R.; Ramsden, D.A.; Rothenberg, E. Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair. Proceedings of the National Academy of Sciences of the U.S.A., 2015, 112(20).Sulfo-Cy3 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For labeling proteins and peptides, no organic co-solvent is needed. Sulfo-Cy3 NHS ester is ideal for proteins with low solubility and proteins prone to denaturation. Non-sulfonated Cy3 NHS ester is also available. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions. In vitro, sulfo-Cy3 NHS ester can react with green-emitting QDs ligand-exchanged and LA/His-PIMA-PEG-NH2 to provide QD-dye conjugates [1].Reference:[1] Reid, D. A.; Keega
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Cy3 maleimide (non-sulfonated)
作者：德尔塔 日期：2022-09-27
Labeling of cysteine residues, as well as other thiolated moleculesCy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other thiolate molecules (such as thiol-containing oligonucleotides). Before the labeling reactions, Cystines should be reduced with TCEP (tris-carboxyethylphosphine). For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. MSNs were labeled by Cy3 maleimide named MSN-Cy3s at the concentration of 10 μg ml-1. MSN-Cy3s incubated with MEF-mEGFP cells on a glass coverslip in culture medium for 2 h To further visualize the MSN locations in the cytosol we used super-resolution microscopy (3D SIM). The visually microscope image results confirm that MSNs were internalized by MEF-mEGFP cells [1].Reference:[1]. Chiu, H.-Y.; Deng, W.; Engelke, H.; Helma, J.; Leonhardt, H.; Bein, T. Intracellular chromobody delivery by mesoporous silica nanoparticles for antigen targeting and visualization in real time. Scientific Reports, 2016, 6, 25019.Cy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other th
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